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Nuclear ER and PR expression levels were quantitatively determined using the aforementioned algorithm.
The width of the PDL-space surrounding the second maxillary molar was determined using the aforementioned buccal and lingual sagittal sections (N = 3 each group).
All the climatic and eddy covariance variables used in this work, except the gross primary production and ecosystem respiration were determined using the aforementioned eddy covariance setups, meteorological instruments and equations.
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Finally, groundwater protection zones were probabilistically determined using the three aforementioned methods, and the estimated accuracy of the delineated groundwater protection zones was gauged using a cross-validation procedure based on observed nitrate-N data.
Each hospital's OPCAB volume was calculated on the basis of the total number of patients who underwent OPCAB, including those with the aforementioned exclusion criteria, determined using the unique hospital identifier.
To define the basis of the aforementioned changes in cell growth, gene expression profiling of each cell population was determined using the Affymetrix GeneChip Mouse Genome 430 2.0 Array.
A secondary tier of lesser significance was determined using just the aforementioned p-value criterion.
Using the aforementioned selection criteria, we determined 3,544 consistent differentially methylated CpG sites associated to mutation in the WRN gene (13,501 for AG11385 and 17,506 for AG07896).
In turn, the average annual cost of extracting a typical board foot of timber from a concession was determined using cost data from the budgets of the aforementioned management plans of reforestation concessions obtained from ProNaturaleza (Supporting Information Table S4).
Enrichment odds ratios and P values were determined using Fisher's exact test and the aforementioned values for the sizes of the P5-Up and P5-Down gene sets and the expressed RPE cell genome.
Relative gene expression of the aforementioned genes was determined using a 7500 fast real-time PCR system (Applied Biosystems) with SYBR Green reagents.
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