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At the determined time interval, samples were taken and mixed with trifluoroacetic acid (TFA) and acetonitrile to precipitate proteins, respectively.
In [1], techniques are proposed to maintain sequence-based windows where the number of data elements in the window is fixed and time-based windows where data elements belong to a determined time interval.
For this purpose, each node within a determined time interval, periodically broadcasts a short beacon packet to announce its presence and geographical position information to those of its neighbors that are within its transmission range.
The bladder was emptied prior to beginning each new short collection; thus, each short collection represented the total volume of urine produced during a determined time interval.
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At determined time intervals, a sample (100 μL) was taken for RP-HPLC analyses.
Decay chains can be simulated under continuous and successive nuclide mass flow events into and out from the system and in freely determined time intervals.
Intragroup statistical analysis for the determined time intervals was performed with the Wilcoxon matched-pairs signed-rank sum test.
Intergroup statistical analysis for determined time intervals was performed using the Mann–Whitney U test for continuous variables and using the Pearson chi-square test for dichotomous variables.
We determined time intervals based on the minimal BMP6 treatment durations needed for significant phenotypic changes, including the expression of osteoblast markers and formation of mineralized extracellular matrix.
The sample was placed on the surface of the wound model, the Petri dish was covered with a lid to prevent water evaporation, and swollen films were then weighed at determined time intervals (W s ).
The Petri dish was covered with a lid to prevent water evaporation, and surface pH was measured at determined time intervals in triplicate on both sides of the film.
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