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The physiological state of the three strains was also determined through flow cytometry using propidium iodide and 3′-dihexylocarbocyanine iodide thus gathering information on their viability and activity with the three oxygenates since previous microbial counts revealed slow growth.
Induction of the synthesis of the fluorescent reporter protein DsRed.T4 was determined through flow cytometry and the corresponding functional titers were expressed as gene switch-activating units (GSAUs) per ml.
To evaluate the effect of Tan IIA on cell cycle progression, the cell cycle distribution was determined through flow cytometry analysis.
CD3, CD4, CD8, CD19, CD11c, MAC-3, CD45, CD34, CD73, SCA-1 Ly-6AE) expreSCA-1 Ly-6AEexpressionthrough floweretometry immunostaining protocol using antibodeterminedthroughh flowrocytometryD Bimmunostaining, USA).
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Th1/Th2/Th17 and Treg cell profiles were determined through ELISA, flow cytometry, and real-time PCR.
At the end of the period, brain/spinal cord histology was performed to measure lymphocyte infiltration; T-cell profiles were determined through ELISA, flow cytometry, and real-time PCR.
NP-induced toxicity was then determined using an alamar blue assay or through flow cytometry utilizing a T cell-specific fluorescent antibody and propidium iodide (PI) dye uptake [16].
They were then passed through flow cytometry to ascertain purity.
A recent survey of Arabidopsis accessions through flow cytometry suggested variation in genome size [ 28]; it was not determined whether RNV or CNV was associated with these changes.
Macrophages were immediately processed through flow cytometry.
The stained cells were analyzed through flow cytometry.
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