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We generated constructs expressing mutant seipin proteins and determined their expression and localisation.
In addition, we developed antibodies against MGL and ABHD6 and determined their expression in autaptic cultures.
In this study, we identified 26 RTK oncogenes in the channel catfish genome, and determined their expression profiles after ESC infection and hypoxic stress.
We tagged all 27 Drosophila Rabs with YFPMYC at their endogenous chromosomal loci, determined their expression and subcellular localization in six tissues comprising 23 cell types, and provide this data in an annotated, searchable image database.
For the purpose, we performed RT-qPCR for 10 commonly used reference genes from various functional classes and then determined their expression level in MSC from goats intramammary challenged with Staphylococcus aureus and in MSC from healthy controls, with a view to select genes whose stability would be unaffected under infection conditions.
Using the UAS-gfp as reporter, we determined their expression pattern.
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Primers and specific Taqman probes were designed from these sequences to determine their expression by quantitative PCR.
To determine their expression patterns, we first performed expression analyses with transcriptional fusion genes.
At first, we selected 47 larval stage high expression tags to determine their expression levels by qRT-PCR.
Several studies have been undertaken to unravel the role of these cytokines in the pathogenesis of PVR by determining their expression in PVR vitreous and PVR membranes [2] [12].
Thus, appropriate generation of splice variants in the organism must be under the control of cues that determine their expression in different conditions.
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