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First, we determined the interaction between Usa1 derivatives and Hrd1 by co-immunoprecipitations. Interestingly, deletion of the middle portion (i.e. d3Δ) abolished the binding between Usa1 and Hrd1 (Figure 4B) and also reduced the Usa1-Hrd3 interaction (Figure 4D), suggesting that the interaction between Usa1 and the Hrd1-Hrd3 E3 complex is important for ERAD.
As a positive control, we determined the interaction between endogenous procaspase-9 and Apaf-1.
We also identified and cloned five and eight Clade A basic leucine zipper (bZIP) and protein phosphatase type 2C (PP2C) genes from canola and, using yeast two-hybrid and bimolecular fluorescence complementation (BiFC), determined the interaction between individual BnaCPKs and BnabZIPs or BnaPP2Cs (Clade A).
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We have also determined the interactions between these factors and several ribosomal proteins with known binding sites in order to map the protein-protein networks onto the structure of the ribosome.
Based on relevant protein-protein interactions known from the published data, IPA then determined the interactions between the focus proteins and all other proteins in the IPA-associated database.
Given that SOD2 is synthesized in the cytosol and translocates to and exerts its catalytic activity in mitochondria, we determined the interactions between SOD2 and three mitochondrially localized SIRTs SIRT3, SIRT4 and SIRT5 and two cytoplasm SIRTs SIRT1 and SIRT2.
Sea ice determines the interaction between marine and terrestrial species, influences ocean productivity, and affects local weather.
The hydrophobic effect determines the interaction between hydrocarbons and water (or lack thereof).
To determine the interaction between ROS generation and Fas activation in regulation of LPS-induced lung epithelial A549 cell apoptosis.
To determine the interaction between histological chorioamnionitis and unexplained neonatal cerebral palsy among low birth weight infants.
In Experiment 2, the goal was to determine the interaction between l-Glutamine and glycerol on ram sperm freezing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com