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The patient exams were performed using a dynamic acquisition mode, with a frame duration of 30 s per frame and a total acquisition time of 8 min. From the acquired data, we determined the background count rates, hotspot contrasts and hotspot sizes for different acquisition times.
We first determined the background level of malformed legs in RhoGEF211-3b/+ RhoGEF211-3b/+ andlts.
As DNA methylation is tissue specific, we determined the background methylation in two non-neoplastic brain extracts, which showed average methylation of <5%.
In this study, the 95th percentile of the R 2 distribution for unlinked markers (markers from different chromosomes, Additional file 1: Table S7) determined the background level of LD (R < 0.091).
We determined the background value that would correspond to an intermediate level of gray between black and white, and for experiments 3 and 4, to several intermediate shades of gray.
However, in order to relate to empirical phylogenetic analyses (where these rates are not routinely determined), the background in this figure has been color-coded based on the percentage of alignment gaps (which can be easily determined), and the contour lines of phylogenetic accuracy have been drawn against this background.
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'vtpdetect' determines the background flux cutoff.
Parallel control experiments were run to determine the background metal contamination in the materials used.
To make the exposure time estimate one needs to determine the background count rate, b.
This helps to determine the background and nonspecific hybridization that contributes to the signal measured for the perfect match oligo.
The key challenge in deriving a photometric light curve from ACA monitor window data is determining the background dark current.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com