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To assess the pharmacodynamics of Nrf2 activation by TBE-31, we determined the activity of NQO1, an enzyme encoded by a prototypic Nrf2-dependent gene, in liver and heart of the animals.
To determine if a residue's contribution is independent of or dependent on the other side chains, we determined the activity of a minimal form of the AP active site with all five residues mutated, herein referred to as AP minimal (D101A/D153A/R166S/E322Y/K328A), and we also restored each side chain individually to this minimal enzyme.
To determine whether caspase activation is involved in Cre-induced cardiomyocyte apoptosis, we determined the activity of caspase 3.
In the first set of experiments, we determined the activity of wt hAID and its mutants on the 80-nucleotide ssDNA containing the AGCT motif.
We determined the activity of the collagenase/gelatinase enzymes produced by these microorganisms when grown on pig skin, which was used as a surrogate for human skin.
Firstly, we determined the activity of [177Lu]-DOTATATE-induced nephrotoxic effects measured with MAG3 scintigraphy in mice.
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Therefore, we determined the activities of H+-ATPase and Ca2+-ATPase in plasma membrane and pH of nutrient solution.
We determined the activities of selected signaling molecules and pathways that impact PC function.
The supernatant was transferred to a pre-cooled centrifuge tube and determined the activities of caspases immediately.
Since gemcitabine cytotoxicity is dependent on its phosphorylation, we determined the activities of the enzymes involved in this process.
We used fibrin zymography to determine the activity of the plasminogen activators as previously described [48].
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