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We have determined that a decrease of O2 concentration to 3% was sufficient to induce HIF-1 activation in MCF7 cells.
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By comparing the experimental depolarization-evoked peak [Ca2+]i elevations in the control and db/db beta-cells pretreated with TG, we determined that a 27% decrease in Ca2+ influx from db/db cells was required to account for the decrease in amplitude triggered by depolarization.
For example, Landolt and colleagues [ 1] determined that QOL significantly decreases during treatment for childhood cancer, but most aspects of QOL improve as children move off treatment.
Furthermore, Huang et al. determined that these effects are due to a decreasing of endogenous reactive oxygen species.
A previous study had already determined that a pnp mutation decreased the motility of the foodborne pathogen Campylobacter jejuni [ 26].
Quantitative RT PCR determined that expression of Dsp and Calb1 decreases in the mutant by a factor of 14.7 (P<0.005) and 4.8 (P<0.05), respectively.
We have determined that the decrease in PI3K/Akt signaling as a consequence of culture in differentiation media is transient, with signaling levels rising again after 6 h [ 55].
Using the Cell Proliferation ELISA, BrdU (colorimetric) kit (Roche), we determined that this decrease in cell viability was the result of a down-regulation in cell proliferation, as opposed to an increase in apoptosis.
We determined that the decrease in gonocyte number in MEHP-exposed testes resulted from a combination of decreased proliferation and increased apoptosis.
By using the external cause of injury mortality matrix, we determined that the decrease in the mortality trends of some unintentional injuries might be due to the increase in mortality trends of the same mechanism of injury with an undetermined intent (Lu 2002).
Using the kinetics model for sensitivity analyses, it was determined that the decrease in reactivity in the presence of H2S is because H2S initially reacts before the H2 fuel does, mainly through the reaction H2S + H ⇄ SH + H2, thus taking H atoms away from the main branching reaction H + O2 ⇄ OH + O and inhibiting the ignition process.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com