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To understand mechanistically how the F-BAR domain functions, we determined its structure, elucidated how it interacts with membranes, and identified an interaction between dimers that allows helical oligomerization and membrane tubulation.
Although its size has been determined, its structure and the types of units needed have not.
To understand the role of the WD40 repeat domain of Prp19 we have determined its structure using X-ray crystallography.
Using the Clover structure, we rationally engineered a non-photoactivatable redox sensor, roClover1, and determined its structure as well as that of its parental template, roClover0.1.
Here, we have identified an additional conserved domain C-terminal to the SANT-SLIDE domain and determined its structure by multidimensional heteronuclear NMR spectroscopy.
We identified a soluble expression construct of the IcsA/VirG α-domain using the ESPRIT library screening system and determined its structure to 1.9 Å resolution.
Similar(41)
Therefore, to understand how a protein works it is important to determine its structure, but this is very challenging.
As HOBB shows a keto-enol tautomerism (Stope et al. 2002), it is difficult to directly determine its structure by GC MS and NMR.
Defining your goals for the site will help determine its structure and the kind of content it contains.
"When he started working on the [large] subunit,... a large and well-funded group in Germany that had first crystallized it had been working on it for years and made little progress on determining its structure.
The structural integrity of the E423A mutein was then confirmed by determining its structure to 2.3 Ǻ resolution after soaking a crystal with maltose (PDB entry 4CN6 in Table 1 and Figure S1D of the Supporting Information).
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