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Electrostatic calculations were carried out using either the Charmm atomic radii or a set of optimized radii determined initially for proteins and augmented for nucleic acids [51], [52]; in the latter case an Mg <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0009142.e189.PNG" class= inline-graphic"/> radius of 1.55 A was assigned.
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The DNA sequences outside exon 2 were not determined initially.
Ethnicity was determined initially by last name.
This gene order, initially determined for Drosophila yakuba mtDNA, is considered ancestral for the entire class Insecta [ 5- 7].
The initial distance walked is initially determined for each individual based on how far the person can ambulate before visibly becoming fatigued or short of breath, reporting pain or requesting to sit down or rest.
Land demand is initially determined for each scenario on the basis of factors exogenous to the land allocation block, but land allocation outcomes are fed back into the demand block so that the system is modified dynamically as RE implementation progresses.
The drug that was used initially determined exposure status for the entire follow-up period.
Protein content of cell culture wells was initially determined to be equal for all treatments.
To better understand the relatively small effect of the exonuclease activity on accumulation of mismatches, we initially determined the optimal substrate for the exonuclease.
We could not initially determine G type for 10 RAV and P type for 8 RAV isolates, even though their VP7 and VP4 genes were successfully amplified by RT-PCR.
Data have been fitted with the direct-impact model and recrystallization cross-sections and threshold values for recovery have been determined for both types of initially defective structures.
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