Bmi1 downregulation induced cell differentiation associated with morphological changes and decreased expression of the stem cell-related proteins Nestin and Sox2, accompanying induction of an astrocytic fate in U373 glioma cell line, determined by increased levels of the astrocyte-specific marker GFAP, and decreased levels of oligodendrocyte-specific marker CNPase (Figures 2A D and S2B).
We next determined whether increased levels of bcl-2 expression in MCF7/BP1 cells could be attributed to direct regulation of the bcl-2 gene by BP1 protein.
Further studies of target organs, such as the lungs, will be needed to determine if increased levels of these cells correspond with pulmonary infiltration.
To determine if increased levels of cAMP could reduce the TGF-β1 induced levels of α-SMA, forskolin, a well-established adenylyl cyclase (AC) activator and an inducer of cAMP in fibroblasts [ 20, 24, 25] was utilized.
To determine if increased levels of IRF4 in pro-B cells trigger premature rearrangement, we purified primary pro-B cells from PIP transgenic as well as from NTG by flow cytometry and analysed the levels of recombination.
To determine if increased levels of IGF-I can initiate mammary tumorigenesis and/or promote ErbB2-induced tumorigenesis, we crossed TTR-IGF-I transgenic mice with the well characterized MMTV-ErbB2 transgenic mice.
Next, we determined if the increased levels of AIB1 and CBP coactivators in 436.1 cells modified the levels of transfected ER-α.
A selenite response in B. napus necessitates the reconfiguration of primary metabolism, as determined by the increased levels of amino acids and the decrease in many TCA cycle metabolites.
The hypoxia-inducible factor (HIF) signaling pathway was found to be activated in D-HF patients, determined by the increased levels of HIF 1α mRNA in D-HF biopsies when compared to ND-HF.
To determine if these increased levels of neurogenesis-related protein resulted also in increased amounts of newborn cells, 6-month-old mice were treated with the base-analog BrdU during 7 days and sacrificed 14 days later.
