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Changes of gene expression of IL-1, IL-4, and IL-12 in which their levels were under the limit of detection of ELISA in BALF (Table 2), were also within 2-fold and not determined in this analysis (Table 3).

Figure 5 (a) Modeled fractional abundance of arsenite relative to thioarsenite species as a function of pH and sulfide concentration based on formation constants determined in this study (Table 4).

Figure 3 (a) Distribution of arsenite and thioarsenite species at pH 7 as a function of the concentration of bisulfide based on the formation constants determined in this study (Table 4).

The activity of the AChE within 72 hpf was quantitatively determined in this experiment (Table 2).

The total soluble proteins in all parts of the cotton seedlings were also determined in this study (Table 4).

We included the novel sequences determined in this study (Table 1), and sequences identified by the BLAST program from the Galdieria sulphuraria genome database [ 22], the Joint Genome Institute [ 23] and the Acanthamoeba castellanii EST database in TBestDB [ 24].

Specific viral load type values were also determined in this study; Table  3 gives both absolute and normalised viral load values for each type infections at the start of the study.

DNA sequencing was performed as described using M13 universal primers and internal sequence primers designed from the sequences determined in this study (Supplementary Table S1).

A summary of the crystal structures determined in this study is in Table 1.

The statistical comparison of ampicillin, with the highest rate of resistance, to ciprofloxacin, with no resistance rate, was also determined in this study (P <0.0001; Table  2).

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