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Unfortunately, in the available studies where both mitotic activity and gene expression have been determined in the same tumor sample [4], [11], the mitotic activity was not measured by protocols of proved reliability [38], preventing a direct comparison.
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To investigate a possible interrelation between miRNA-218 and GLCE expression, GLCE mRNA and protein levels were determined in the same breast tumors and normal tissues by TaqMan-based qReal-time RT-PCR and western blot analysis, respectively (Fig. 2A and B).
Mean HU, maximum HU, and primary tumor size were simultaneously determined in the same manner.
MSI status of the tumors in the validation series was determined in the same manner as for the test series.
mRNA expression in SFM was determined in the same way.
The boundary correction Y was determined in the same manner.
A (absent) detection calls were determined in the same way.
We looked for CIS-CIS interactions by determining whether particular CISs are found mutated together in the same tumor at higher or lower rates than expected by chance.
Three samples (8.8%) had both 15 9 and 16 9 fusion genes identified in the same tumor.
Distinct molecular scores could be determined from different cores of the same tumor specimen.
Moreover, the expression of MDM2 and CDK4 in whole tissue sections was compared with that in core-needle biopsy sections of the same tumor in order to determine whether real-time PCR could be used to distinguish ALT/WDL from lipoma at the preoperative stage.
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