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Affinities of Oxa for the other biologically important ions such as Ca2+ (Kd1="250 μM and Kd2= 275 mM) and Mg2+ (Kd1= 975 μM and K2d could not be determined) in the same conditions were also obtained.
The susceptibility of this mutant to MMTS was determined in the same conditions as those of the other chimeras and the result showed that it had an intermediate susceptibility between WT TbTIM and WT TcTIM (Figure 3).
For quantification, a relative standard curve was determined in the same conditions for each target gene with a fourfold dilution (from 100 to 0.097 ng) of a cDNA template mix.
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In order to determine if the same condition of hypoxia affected other types of cancer growth, we placed rats with colon cancer tumor in the same chamber and found that the growth of colon cancer tumor was significantly stimulated.
The kinetics of the RR120 adsorption on the four clays [Fouchana clay, smectite (SWy-1), kaolinite (KGa-2) and illite (IMt-2)] determined in the same operating conditions showed rapid dye removal in the initial stages of contact time and reached equilibrium after 20 min for Fouchana and KGa-2, and after 60 min for SWy-1 and IMt-2 during a 180 min reaction time.
The measuring determined that in the same conditions, various materials yield various maximum temperatures.
Body condition scores of cows were determined in the same time periods.
The affinity of the tagged IP3R1 expressed in Sf9 cells is similar to that determined under the same conditions for native IP3R1 in cerebellar membranes [ 12] and of untagged IP3R1 expressed in Sf9 cells [ 23, 39, 40], and the level of expression is ~40-fold higher than that of native IP3R in Sf9 cells [ 40].
The amount of polyubiquitylated proteins and the rate of turnover of polyubiquitylated proteins (see the HMW protein species marked by *) were determined under the same conditions as in Fig. 1B using the anti-HA antibody in immunoblotting.
The amount of polyubiquitylated proteins, and the rate of turnover of these polyubiquitylated proteins (see the HMW protein species marked by *) were then determined under the same conditions as in Fig. 1A, except that anti-HA, rather than an anti-ubiquitin antibody was used in immunoblotting.
In addition, mRNA expression levels were determined for the same conditions using Affymetrix Human Genome U133 plus 2 GeneChip [22].
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CEO of Professional Science Editing for Scientists @ prosciediting.com