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The membrane IV characteristic was determined in the presence of CCCP (50 nM) or indole (2.5 mM) (Fig. 3c, d).
Nonspecific binding was determined in the presence of 1 μM spiperone.
Nonspecific binding was determined in the presence of 5 (1 μM) as a competitor.
Non-specific [3H]DA uptake was determined in the presence of 10 μM nomifensine.
Nonspecific uptake was determined in the presence of 10 μM final concentration of nomifensine.
For instance, the Tm of 2AP-LC13 was determined in the presence of matching RC14 and cross-binding LC23 (Fig. S2).
Since HQ requires a reducing agent in order to become a TyrBm substrate, the kinetic parameters of the monophenolase activity were determined in the presence of H2O2.
The GA is determined in the presence of various inorganic ions and biological species especially adenine.
Then, the influence of sulfate ions is determined in the presence of another anion.
In addition, the in vitro cytotoxicity of these copolymers was determined in the presence of L929 cells.
In this method the solubility of a basic drug is determined in the presence of various salt-forming acids.
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