Sentence examples for determined in live from inspiring English sources

Autophagic flux was determined in live cells using Cyto-ID autophagy detection kit as described previously52.

Mitochondrial mass was also determined in live cells using the specific mitochondrial probe MitoTracker Green FM.

The cellular localization of the YFP- Blm10 fusion protein was determined in live cells by fluorescence microscopy.

In Figure 5, after gating on CD4+ cells, CD25− and CD25+ subsets were determined in live and dead cell gates (Supplementary Figure S2).

Transfection efficiency was determined in live cells by counting, with a fluorescence microscope fitted with phase contrast optics, the number of GFP-positive cells as well as the total cell number in 10 independent optical fields.

E-cadherin expression was determined in live cells by mouse antibodies against human E-cadherin (Santa Cruz Biotechnology, Santa Cruz, CA), followed by FITC-conjugated antibodies against mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania).

Thus, using this approach, the fate of single altered cells that are surrounded by wild-type cells can be determined in living animals.

ROS levels were determined in living cells using the Image-iT LIVE Green Reactive Oxygen Species Detection Kit (Molecular Probes, Eugene, OR, USA), which uses carboxy-H2DCFDA (5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein 5-[and-6-]-carboxy-2′,7′-dichlorodihydrofluorescein

The meat quality phenotype established later after slaughter depends on the transcriptome of skeletal muscle prior to slaughter and thus is already determined in living cells under genetic control.

Image resolution does not need to improve on that of the electron microscope (at the cellular level) and X-ray crystallography (at the molecular level), but without doubt the new abilities to determine in live or un-fixed specimens, the exact position of identified molecules in complex molecular/cellular machines will be utterly fascinating (Wong et al., 2014).

Taking into account our previous assessment of the interaction of rGKRP with human GCK by FRETN analysis in COS-1 cells [ 21], direct interaction determined in our experiments in living cells appeared to be strongest between hGKRP and GCK.