We determined generation of thrombin-antithrombin complexes (TATs) and soluble CD40 ligand (sCD40L), a platelet activation marker, at the site of microvascular injury, together with ex vivo plasma fibrin clot permeability and lysis time.
The sonication conditions were optimized to determine generation of DNA fragments between 300 and 600 bp in length.
Since the cost of natural gas is high, the principle of determining generation based on heat demand is applied.
We determined the generation of reactive oxygen species by peritoneal neutrophils as determined by flow cytometry using dihydrorhodamine-123 to detect intracellular ROS.
To rule out this possibility, we determined the generation of DSBs in MIG-R1 and MIG-210 transduced CB CD34+ cells by means of the analysis of γ-H2AX nuclear foci.
The 95% confidence intervals (CI) for the QTL location and effect were determined by generation of 200 bootstrap samples.
To assess the direct effects of various PAMPs on the metHb-POX activity, we incubated 4 μl/100 μl of metHb with 25 μl/100 μl of LTA or CpG DNA (ODN2395) for 30 min, and then determined the generation of O2− (ROS).
To attempt to minimize the variability in estimates of short-term plasticity stimulation was performed at the half-maximum stimulus intensity determined by generation of an input output function at the beginning of the experiment.
The relationship of the AH2012 strain with the 33 PEDV strains identified in China, the United States, South Korea, and Belgium (Technical Appendix Figure 1) in nonstructural protein genes was also determined by generation of 3 phylogenetic trees based on ORF1ab, ORF1a, and ORF1b genes, respectively.
To further determine whether generation of ROS can regulate apoptosis, N-acetyl-L-cysteine (NAC), a precursor of reduced glutathione (GSH) widely used as a thiol-containing antioxidant to scavenge intracellular ROS, was added before administration of Lexa and CHX.
