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Percent frequencies of the different combinations of CD107a, IL-2, TNF-α and IFN-γ+ cells following antigenic stimulation were calculated within the total population of CD4+ or CD8+ T cells and background subtracted (as determined from the medium alone control).
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Data are expressed as percentage of total LDH activity, after subtraction of background determined from the serum medium alone.
The optimized medium, determined from the experiments described above, was used for the rest of the study.
NO production by cultured EPCs can be determined from the NO content in the culture medium [ 18].
The terminal velocity of dense particles in an air-fine particle medium was experimentally determined from the turbulent fluidized bed voidage vs air velocity relationship in this study.
The relative densities, I(_mathrm{D} k)) are determined from the correlation defined for sands of low, medium and high compressibility, respectively.
In the first block of stimulus presentation (Fig. 1A, left, "mixed contrasts"), the contrast of the grating could take, randomly, one of the three values (low, medium and high contrast) determined from the CRF.
The amount of TGF-β1 production was normalized relative to viable cell numbers determined from the MTT assay after subtracted the value of culture medium.
Spontaneous release was determined from the supernatants after labeled cells were incubated in 0.1 ml of medium without effector cells.
Concentrations were determined from the standard curve.
RNA concentrations were determined from the A260.
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