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Next, we determined expression of the Sulfs in human pancreatic adenocarcinoma cell lines by using RT-PCR to survey a panel of 24 cell lines.
In this study, we determined expression of the total leptin receptor (ObR) as well as ObRb.
We first determined expression of the myc-tagged mutant forms using western blot analysis of embryo lysates.
We also determined expression of the epithelial and mesenchymal cell markers, cytokeratin 8 (CK8) and vimentin, respectively.
Western blotting and immunohistochemical (IHC) staining determined expression of the autophagy markers Light Chain 3 (LC3) and beclin‐1.
We determined expression of the insert region and flanking operons by mapping the quality-filtered reads back to the plastome reference sequence using BWA.
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We therefore set out to determine expression of the target genes that we have identified.
To confirm this hypothesis, RT-PCR analysis was performed to determine expression of the early mesodermal marker (BrachyuryT and Tbx6), cardiomyocyte-specific transcription factors (Csx/Nkx2.5), structural genes (β-MyHC), and Gapdh (Fig. 4A).
Immunohistochemical staining (IHC) was performed to determine expression of the ER and progesterone receptor (PR).
Determining expression of the calpain system may be of benefit in patients with pancreatic, bile duct of ampullary cancers.
Total RNA was isolated from the brain, spinal cord and thymus of transgenic mice to determine expression of the transgenes.
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