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The luminescence signal was determined every hour for 72 h from the same assay wells.
The fish were closely monitored and mortality was determined every hour.
The fate of such millions of DNA damaged cells is determined every hour, and even a modest influence of higher IGF-1 level on survival probability might lead to an association of circulating level with cancer risk [ 146].
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The algal biomass was determined every 24 hours for 72 hours based on cell counting using a hemocytometer under a microscope.
The increase in cell number was determined every two hours.
Parasite density was microscopically determined every 24 hours for 7 to 14 days.
Before or during each experiment, parasite density was microscopically determined every 24 hours using Uriglass disposable count chambers (Menarini diagnostics) to follow up the growth and differentiation profile.
In brief, MLTCs were cultured in 96-well plates (an initial cell count: 1×104 cells/well), and the cell number was determined every 24 hours by measuring the absorbance on a plate reader (Molecular Device) at 490 nm.
Infection experiments with the entomopathogenic fungus Beauveria bassiana (Boverol®, Fytovita, Ostrožská Lhota, Czech Republic) were performed by injecting 104 conidia spores into the hemocoel of imagoes, and survival rate was determined every 24 hours at 25°C.
To determine whether the unmarked Schu S4 ΔpurMCD mutant was also attenuated for growth in human cells, human peripheral blood monocyte-derived macrophages (hMDM) were infected with this derivative at a multiplicity of infection (MOI) of 1, and bacterial burden determined every 16 hours for two days.
Urine specific gravity was determined every 24 hours until discharge.
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