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I think that if the two remaining contenders are John McCain (fiercely independent) and Barack Obama (determined consensus builder), the country might be in store for a refreshing change in conversation as to how do we fix our problems together from how do we create more problems through alienation.
By Sanger sequencing, we determined consensus whole-genome sequences for A/H1N1pdm viruses sampled nationwide in Canada over 33 weeks during the 2009 first and second pandemic waves.
Restricting the analysis to changes in experimentally determined consensus motifs bound by well-known trans-factors (SC35, SRp40, SRp55, SF2/ASF and hnRNPA1, see Methods) also showed no association (Table 2), although the number of changes observed in these motifs is too small to reach confident conclusions.
From the resulting 291 groups, which approximately correspond to the microRNA families, we determined consensus sequences.
Binding sites were identified using local perl scripts based on the previously determined consensus sequences [ 37].
We have determined consensus sequences for the putative promoter regions (Boxes A and B) of the Tritryps tRNA genes, and specific changes were found in tRNA-Sec genes.
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In an effort to determine which assessment best represents the experimental data, weighted means statistics are used to determine consensus estimators for the data.
The Delphi method is largely used in the healthcare setting as a reliable means of determining consensus for a defined clinical problem [28 30].
Although we were able to align and determine consensus primer sets for the 9 tri5 and the 16 β-tubulin sequences, there was no consensus sequence that could be derived from alignment of the 11 rRNA sequences.
Ten to twelve clones were sequenced per PCR product to determine consensus sequences [16].
We feel, however, that weighing individual amino acids in their contribution to overall peptide array phosphorylation seems a valid tool for determining consensus sequences.
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