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The NP-PCM's are based on commercial poly vinylidene fluoride), with variable pore size determined by the addition of nanoscale SiO2 or TiO2 particles.
In the first step and during solution preparation, those impurities are separated which can be precipitated by iron, or sorbed in the resin at low HCl solutions under reducing conditions determined by the addition of Fe powder.
Protein content was determined by the addition of a 100-μL sample to a 3-mL Bradford reagent.
The FAM-labeled PCR products were separated on an ABI 3130xl and the size was determined by the addition of ROX-250-labelled size standards (ABI).
24 hours after treatment, media was replaced with serum-free media and cell viability was determined by the addition of a tetrazolium salt solution, WST-8 (Dojindo, Indianapolis, IN) to each well according to manufacturer's protocol.
After the maximum incubation time for these assays (4 24 h), cell viability in comparison to untreated control cells was determined by the addition of MTT as described for cell growth assay.
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They are determined by the addition position of X and not by the chemical nature of X.
Acidity and/or neutrality of goblet cells are determined by the addition or removal of sugars on mucins by transferases.
The acidity of goblet cells is determined by the addition and removal of sugars to the mucins within the goblet cell [ 42].
This is because, for orthogonal components, the components of the vector sum are uniquely determined by the scalar addition of the components of the individual vectors.
The phosphorus concentration was determined photometrically by the addition of ascorbic acid and ammonium molybdate at 880 nm.
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