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Topical application of 0.05% RAL for 3 days resulted in a significant epidermal hyperplasia and keratinocyte proliferation as determined by quantitation of Ki67 in the back skin of SKH1 hairless and DBA/1 mice, whereas no epidermal hyperplasia and keratinocyte proliferation was observed in CD44−/− mice.
The cross-sectional area of β-cells was determined by quantitation of the area occupied by fluorescently labeled β-cells (green; all fields with β-cells present, a minimum of 20 images) and the cross-sectional area of all tissue visualized by DAPI (blue fluorescence).
Similar data were obtained on the basis of the proliferation index of tumours as determined by quantitation of MIB (Ki-67) positive cells.
Equal loading of the sample was determined by quantitation of protein as well as by reprobing membranes for β-actin (Imgenex, San Diego, CA) as a housekeeping protein.
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Changes in Nrf2 protein expression were determined by quantitation from Western blots of the 3 WT lines, with each Nrf2 band adjusted for the loading control β-actin.
RNA-seq-based mRNA quantitation was strongly correlated with gene expression levels determined by quantitative RT-PCR.
The third metric, recovery efficiency, is determined by Pico Green quantitation of DNA following Proteinase K digestion of the recovered tissue.
Id1 and integrin α4 mRNA in the EPCs was determined by relative quantitation, interpolating from a standard curve of template DNA of known concentration and then normalized using β-actin as an internal control.
VP-16-induced changes in apoptosis were determined by flow cytometric quantitation of cells in sub-G0/G1 fractions, which was confirmed by TUNEL analysis (see Additional file 4).
A final yield of 37.3 mg of panitumumab F ab' 2 was obtained as determined by protein quantitation by the Lowry method.
The amounts of NADP+ and NADPH were then determined by NADP/NADPH Quantitation Kit according to manufactory instruction (Biovision, CA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com