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The mean residue molar ellipticities of the peptides were determined by circular dichroism (CD) spectroscopy of J-810 spectropolarimeter (Jasco, JAPAN) with a 0.02-cm path length quartz cuvette at 25°C as described previously (Huang et al., 2010a).
We show that this high-quality approach translates very well to the CaM protein scaffold: All library members over-express and are functionally diverse, having a range of conformations in the presence and absence of calcium as determined by circular dichroism spectroscopy.
Protein secondary structure content was determined by circular dichroism (CD) spectroscopy.
The midpoint of thermal denaturation of proteins was determined by circular dichroism.
Thus, the FC-14 solubilized Trx-hCRs could be purified using size exclusion chromatography as monomers and dimers with the correct apparent MW and their alpha-helical content determined by circular dichroism.
Deacetylase activity of HP0310 in solution was assayed in three ways: for substrates without a free amino group before the reaction, a fluorogenic test was used; for all the others, except allantoin, by determining the amount of acetate liberated from the substrate during the reaction using an acetic acid kit (R-biopharm); allantoinase activity was determined by circular dichroism.
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The absolute configurations of these chiral molecules were determined by a circular dichroism method.
The absolute configuration at the C1 position of the synthesized compounds was determined by the circular dichroism exciton chirality method using the corresponding C1-allylic benzoates.
The absolute configurations at the C4-hydroxy groups of the targeted compounds (3a,b) were determined by the circular dichroism (CD) exciton chirality method using the corresponding benzoates of the C4-allylic alcohol.
The exact degree of activation is determined by the circular Gaussian tuning curve associated with each presynaptic cell.
The stability of the WW domains was determined by equilibrium circular dichorism and Fourier transform infrared (FTIR) measurements.
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