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The degenerate primers were designed according to the codon usage, determined by analyzing a number of different genes of Rhizopus species.

The analytic sensitivity was determined by analyzing a half-logarithmic serial dilution of the IVT ranging from 100 to 0.1 copies/reaction.

mRNA expression levels were determined by analyzing a published gene expression dataset for six human and five chimpanzee livers with added expression data taken from one chimpanzee individual [14].

Enrichment significance was determined by analyzing a hypergeometric distribution as described previously [ 27].

Acceptable reproducibility/accuracy was determined by analyzing a laboratory reference sample of pooled fish oil.

Assay accuracy was determined by analyzing a nonsmoker's urine to which increasing amounts of [D4] 4 were added, ranging from 11.3 fmol/mL to 1.12 pmol/mL.

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The light, intermediate and heavy dimethyl labeled samples were mixed in 1∶1∶1 ratio based on total peptide amount, determined by analyzing an aliquot of the labeled samples on a regular LC-MS run and comparing overall peptide signal intensities.

This was determined by analyzing all actual arm's-length transactions in this neighborhood for each year.

Equations for competitive, uncompetitive, and noncompetitive inhibition were fit to this plot using GraphPad Prism, and the inhibition model was determined by analyzing statistics using an F test.

The reproducibility of the assay was determined by analyzing 3 replicates in a single run for intra-assay precision and 3 independent replicates for inter-assay accuracy.

Figure 5 shows the average compositions of the amine degradation samples determined by analyzing the 250 °C AMS A comparison to the spectra acquired at 600 °C are shown in Figure S7.

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