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A consensus sequence was previously determined by alignment of the promoter regions of 13 PlcR-controlled genes [11].
Four domains in the SVCV-C1 G protein were determined by alignment and comparison of the secondary structures with PyMOL software (DeLano Scientific, http://www.pymol.org).org
Initially, the putative ovine genomic structure was determined by alignment of bovine PITX3 EST and human PITX3 mRNA to the determined ovine genomic sequence using Spidey [51].
The consensus sequence for each gene fragment was determined by alignment of the forward and reverse sequences using BioEdit version 7.0.5.3 (http://www.mbio.ncsu.edu/BioEdit/bioedit.html).html
Unique regions of the contig sequences of N. sicca 4320 were determined by alignment with the contig sequences of the other identified commensals using Nucmer, a component of the Mummer software package [56].
In contrast to a recently published study describing the in vivo characterisation of tail swap proteins, where the C-terminal domain boundary was determined by alignment [27], the constructs described here have boundaries chosen based on those determined by limited proteolysis [28].
Similar(33)
The complete sequences were obtained on both strands using several primers as described previously [ 35] and genomic organization of bovine SERPINA3 genes was determined by alignments using the Sequencher™ 4.1.4 software (GeneCodes Corp., Ann Arbor, USA).
Sequence identity was determined by pairwise alignment using the DNAMAN software package (optimal alignment; gap open penalty 10.0, gap extension penalty 5.0).
Discrepant nucleotides were substituted with the most frequently employed nucleotides, as determined by consensus alignment.
The putative domains of irrE from D. radiodurans were determined by sequence alignment with irrE from D. deserti as described (see Figure 9).
The positions of the markers on the scaffolds were determined by BLASTN alignment.
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