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The total number of upregulated (upper) or downregulated (lower) transcripts identified by mRNA-Seq were determined at various time points of stress (early, middle, and late) during Pi starvation (−P) or phosphate over-abundant Pi supply (++P) treatments.
Due to dynamic nature of AF633-Trf internalization, the co-localization of F-Aβ40 with the endocytotic marker was also determined at various time points of 15, 45, and 60 min following the incubation.
The insoluble polymeric fraction was determined at various time points throughout the SSF experiment.
Cell proliferation was determined at various time points using the sulphorodamine B (SRB) assay (Sigma-Aldrich).
To investigate whether ascorbate activates AMPK, phosphorylation of AMPK at Thr was determined at various time points after treatment.
Cell-cycle distribution of activated PBLs was determined at various time points using a method developed by Vindelöv [ 35].
Similar(51)
The monomer conversion, the polymerization kinetics, the particle size, the particle number density, the poly methyl methacrylate) (PMMA) core diameter, the percentage of unbound PEI, and the grafting efficiency of PMMA were determined at various times during the polymerization.
Concentrations of neopterin, nitrate, tryptophan, IFN-gamma, and tumor necrosis factor alpha (TNF-alpha) were determined at various times during the first 48 hours in 11 patients who received intraperitoneal rhIL-12 in doses ranging from 100 to 1,500 ng/kg.
Histopathology was also determined at various times during the course of infection.
To determine whether 20(OH)D3 affects the classical NF-κB pathway, the cellular levels of IκBα and the p65 NFκB were determined at various times after 20(OH)D3 addition to cells.
ROS levels were determined at various times after treatment and in multiple independent experiments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com