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Supramaximal stimulus intensity was determined at rest by increasing the electrical current until no further increase in twitch torque was observed.
The thickness of the muscle was determined at rest and then with the teeth at maximum contact.
Absolute regional and global myocardial blood flow in mL/min/g can be determined at rest and following pharmacological stress.
Blood lactate was determined at rest, immediately after and 5 min after the 4th exercise bout, with perceived exertion reported immediately after the 4th bout.
Wall Motion Score Index (WMSCI) was determined at rest and peak stress as the sum of the segmental scores of the 16 segments divided by 16.
Post-rest behavior of papillary muscles was determined at rest intervals of 5 and 10 s to biomechanically assess SR Ca2+ content [ 24, 26].
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We did not record mobilization data, and pain scores were determined only at rest.
Resting heart rate (HR) was determined at the end of 15 min, semi-supine rest using a 12-lead ECG (GE Healthcare, Buckinghamshire, UK).
Furthermore, measurement error in determining VO2 at rest and treadmill walking will add to this measurement error.
For measurement of flicker-light-induced vasodilatation RCF was determined at baseline (after 30 minutes of rest) and after flicker light stimulation (10 Hz; Photo Stimulator 750, Siemens-Elema, AB, Germany).
The resting metabolic rate was determined at the Mouse Physiology Laboratory in the Department of Physiology at the Geffen School of Medicine, UCLA using indirect calorimetry as previously described [43], [44].
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