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MEF cell death was determined as the normalized

Thus, the output of each node in this layer is determined as the product of the normalized firing strength and the first-order polynomial function: overline{{w_{i} }} f_{i} = overline{{w_{i} }} ( p_{i} x + q_{i} y + r_{i} ), quad i = 1, 2 (8)where p i, q i, and r i are consequent parameters of rule i. Layer 5 is a summation layer.

Notch activity was determined as the ratio of wildtype to mutant plasmids and normalized to p850-luc and Renilla luciferase activity.

Myometrial gene expression was determined as normalized for expression of 18S ribosomal RNA that we have previously shown to be stable in non-pregnant versus pregnant myometrium [15].

Points x i that are closer (as determined by the normalized weights u i (ψ (s ) ) ) to the current position ψ (s ) have a greater influence on the new position.

For PenV the RMSEP was determined as 0.23 g L−1 (normalized to calibration range: 4.1%), making the performance of the MCR-ALS model similar to the PLS model and the estimated reference method error.

Three independent experiments were performed and the luminescence values were determined as relative units and normalized to vehicle.

The root-mean-square-error of prediction (RMSEP) for POX was determined as 0.16 g L−1 (normalized to calibration range: 4.6%), which is significantly lower than the RMSECV of the PLS-POX model and in the range of the estimated reference method error.

Fig. 3(b) shows the calibration curve for PenV; the respective RMSECV was determined as 0.22 g L−1 and the normalized RMSECV (to calibration range) is 4%, which is in the range of the expected error of the reference method.

Normalized light emission was determined by dividing the FLuc reading by the GLuc reading and normalized TF activity was determined by dividing the normalized light emission for each sample by the average normalized light emission for TA.

We then determined the normalized ECFP/YPet emission ratio in the supernatant samples (Fig. 11A).