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For each strain the number of granules per full size cell picture (ngranules) was determined as well as the total number of full length cells (ncells, or n in formulas 5 and 6).

Naming latencies were determined as the time elapsing between picture onset and the beginning of vocalization and were recorded on line using OPTIMIC™ optical microphone.

Fluorescence microscopic pictures were always taken from five randomly chosen areas and the percentage of cells with fat droplets was determined as described before [ 19, 69].

Proportion of laterally attached (left) or unattached kinetochores (right) at old and young centrosomes in HeLa-eGFP-centrin1/eGFP-CENP-A cells treated with 10 ng/ml nocodazole, as determined in immunofluorescence pictures shown in Figure 3C.

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