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The consistency, initial and final setting times of mixes was determined as per (IS 4031 -1988 part 4 & 5) respectively using Vicat's apparatus.
Metals was determined as per (APHA 1995) first sludge sample was digested with nitric and perchloric acid (3:1) and filtered through Whatman no.
The chemical properties of the Scenedesmus sp. lipids were determined as per standard ASTM methods.
Relative gene expression over control samples was determined as per the comparative cycle threshold (ΔΔCt) method and normalized to the geometric mean of B2M and HPRT reference genes (ΔCt = Ctreference − Cttarget; ΔΔCt = ΔCtXMD8-92 − ΔCtDMSO).
Cyclic variations of material properties for base metal and welded joint zones were determined as per the standard and the methodology presented in previous study and described with the Ramberg Osgood relationship.
COD and VSS are determined as per Standard Methods (1989).
Inhibition of the lipid peroxidation effect of PTVO was determined as per standard procedure [69].
Enzyme activity was determined as per the protocol of Kamarthapu et al. (2008).
Protein concentration in the supernatant was determined as per the method of Lowry et al. ([1951]).
Temperature has to be determined as per the quality of oilseed and fatty acids.
The bound rubber content was determined as per method proposed by the reported literature [30].
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