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After removal of the supernatant, the infected cells were lysed in lysis buffer 1B provided in the cAMP Biotrak EIA System (GE Helthcare, RPN225), and cAMP levels were determined as instructed by the manufacturer.
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They were instructed to determine as quickly as possible whether a target was present or not, but also to make as few mistakes as possible.
I did as instructed.
Ruklick did as instructed.
Do as instructed.
Then do as instructed.
Different concentrations of S100A14 were added to the cells for indicated time intervals, and cell proliferation was determined by MTT assay as instructed by the manufacturer (Roche Applied Science).
Immunomodulation of alloresponses in chimeric animals was determined using the IL-2 ELISPOT assay as instructed by the manufacturer (BD Biosciences).
The Beagle however, would be gone for several years and was required to take chronometers ashore and in boat expeditions up rivers to determine the coordinates of specific reference points as instructed by the Admiralty.
Protein concentrations were determined using the BCATM protein assay kit (Pierce, Bonn, Germany) as instructed by the manufacturer.
Separately global DNA methylation (5-mC) and hydroxymethylation (5-hmC) were determined using two different quantification kits (Epigentek, New York, NY, USA) as instructed by the manufacturer.
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