Sentence examples for determined as before from inspiring English sources

The quantity of protein was determined as before and 100 µg of the mitochondrial fraction was resuspended in respiration buffer (210 mM Mannitol, 70 mM Sucrose, 1 mM EDTA, 4 mM Na2HPO4, 5 mM MgCl2) to determine the rate of oxygen consumption of the isolated organelles using the above Clark electrode system.

The normalised luciferase activity was determined as before and the ratio of wild type to mutant determined for each animal and plotted on a log2 scale (Fig. 3C).

After propagating the transconjugant culture for approximately 50 duplications in VM, the ratio of apramycin resistant cells to total cells was determined as before, and plasmid loss was also calculated.

Senior staff at my and colleagues' hospitals have been supportive of the need of juniors to strike, and the mood seems as determined as before Christmas.

The solution of this model was also an approximate one and only the total biofilm thickness could be determined as before.

Chloramphenicol acetyl transferase (CAT) activity was determined as before [48].

Quantification and size distribution of the final library was determined as described above before sequencing.

He was also determined as never before to distinguish himself beyond his father's legacy.

The endotoxin content was determined as described before.

Raloxifene content of the prepared SMA micelles was determined as described before [13] and expressed as wt% of Ral in the final micelle compared to the total weight of recovered SMA-Ral.

I used to mimic the film's opening shot, sitting with my knees bent in the windowsill of my own little shadowy orphanage, directing myself to appear as "orphanly and determined" as possible before bursting into a pensive version of "Maybe".