Induction of autophagy by YM155 in CAL27 cells was determined according to guideline of autophagy as followed: (a) LC3 dot punctae detection by LC3 immunofluroscence as previously described.
The primary validation parameters were determined according to ICH guidelines and Brazilian legislation.
An employer must be able to demonstrate that the rate being used as prevailing for determining a commensurate wage was objectively determined according to the guidelines contained in this section.
The limit of detection (LOD) was determined according to ICH guidelines for validation of analytical procedures [23] and was found to be 0.49 ng/mL (Table 1).
The limit of detection (LOD) and limit of quantification (LOQ) were determined according to ICH guidelines for validation of analytical procedures [19].
MICs were determined according to CLSI guidelines.
MICs were determined according to CLSI guidelines [48].
Drug doses and schedules were determined according to treatment guidelines and package inserts.
The antimicrobial susceptibility of the isolates was determined according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) [35].
The diagnosis of CD or UC was determined according to established guidelines based on endoscopic, radiological, and histopathological criteria [39].
