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To characterize how residents learn phacoemulsification and determine which steps of the procedure are most difficult to master.
Although chloroplasts have the machinery to produce and assemble these complex proteins with multiple disulfide bonds (Tran et al. 2013a, b), little has been done to determine which steps of complex protein accumulation are rate-limiting.
This permits a broad investigation to determine which steps within glycolysis and what sites within enzymes are the targets of positive selection.
We utilized the approach of analyzing rates of individual reaction steps and measuring KIEs to kinetic models to determine which steps might be limiting, as has been done with several other P450s.
Given the variety of potential responses to outbound medical tourism identified by these stakeholders, ongoing discussion is needed to determine which steps best meet the needs of the entire Mongolian population.
Consider how complex the original picture is to determine which steps you need to follow.
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Antiviral activity of flavonoids quercetin, morin, rutin and hesperidin, and phenolic cinnamic, trans-cinnamic and ferulic acids were evaluated in vitro against the CDV using the time of addition assay to determine which step of the viral replicative cycle was affected.
Future studies are needed to determine which step the mutants are defective in.
To determine which step of deubiquitination is causative, we first measured whether the kinase activities of TBK1 would be directly affected by PLP2 by in vitro kinase assays.
To determine which step in viral replication requires the interaction of virus polymerase with importin α, we reconstituted in vivo RNPs using as replicon a 248 nt long negative polarity RNA derived from viral RNA 8 [18].
We were interested to determine which step of transcription initiation is affected by the deletion of the β′ zipper.
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