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Accordingly, we sought to determine whether the predicted catalytic residues are important for Pmp23 function in vivo and assess the importance of its putative lysozyme activity for pneumococcal division and morphogenesis.
His studies could not determine whether the predicted particle had spin or electric charge, and his paper ends on an apologetic note after he calculates that the particle should weigh about a hundred times the electron mass, or about 1/20 the proton mass.
Expression vectors for these proteins were introduced transiently into HCT116 cells and subjected to western blotting with anti-HA antibodies to determine whether the predicted recombinant CHFR proteins were produced (Fig. 1B).
42 To determine whether the predicted unintended consequences of plain packaging could be detected between 2011 and 2013, we used logistic regression.
34 The protein variability index was used to determine whether the predicted epitopes were positioned in the least variable, moderately variable, or hypervariable regions.
Sequences retrieved were subjected to a TargetP analysis to determine whether the predicted proteins were likely to be localised to mitochondria or microsomes.
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We then used the miRHub algorithm (Methods), which determines whether the predicted regulatory effect of any given miRNA on a specific set of genes is significantly greater than expected by chance (i.e., acts as a "regulatory hub"), to predict whether any of these miRNAs are candidate drivers of the gene expression profiles associated with the phenotypes of interest.
The current study aimed to evaluate the applicability of the methods of Moyers and of Tanaka and Johnston to estimate the mesiodistal widths of permanent canines and premolars in Syrian individuals, to determine whether the predicting equations differ by sex, and to develop more accurate regression equations using various teeth groups as predictors.
PCS becomes, therefore, a criterion for determining whether the predicted transcript should be recorded.
The in silico validation made use of the available A and D diploid genome sequences by determining whether the predicted aa/bb dual homozygous markers had the expected evolutionary pattern for sequences from Scenario 1 (Fig. 1).
Because PH-domain-containing proteins and phosphoinositides can affect Ca2+ signaling, we determined whether the predicted PH domain of murine HADP1 (amino acids 164 184) could function as a bona fide phosphoinositide-interacting motif.
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