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Future cell culture studies are necessary to determine the temporal pattern of TDP-43 and RBM45 deposition within inclusions and if RBM45-containing inclusions are cytotoxic.
To determine the temporal pattern of MBP isoform loss in the Twitcher mouse, spinal cord and sciatic nerves from WT and Twitcher mice at ages P7, P15 and P30 were extracted and homogenized for immunoblot analysis.
To determine the temporal pattern of mitomycin C induced cytokinetic changes, we analysed flow cytometric DNA histograms of CC531 liver tumours from rats treated with high dose mitomycin C (3.2 mg kg-1) via hepatic artery infusion and sacrificed at different time intervals after treatment.
To determine the temporal pattern of expression of the cortactin transgene throughout MG development, the abdominal MG of both T16 transgenic and non-transgenic FVB/N inbred mice were obtained from virgin (V) mice, from mice at day 11 and 17 of pregnancy (P11, P17), from day 4, 10 and 17 of lactation (L4, L10, L17) and from day 4 and 8 of involution (I4, I8).
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Having continuous measurements year-round allows us to determine the temporal patterns of methane emissions.
By comparing different historical periods, it was possible to determine the temporal patterning and characteristics of a stable relationship and to determine when there were deviations from regular patterns of association.
To determine the temporal patterns of gene expression that accompany the induced myopathy, we carried out microarray analysis of skeletal muscles from Tg(HQK) mice, wild-type FVB mice (WT) and PrP-knockout control mice (KO) using a 16,315-gene cDNA array constructed in our laboratory.
Moreover, recent studies on Arabidopsis has shown that determining the temporal pattern of stress responses is critical for distinguishing between genes subject to circadian or diurnal regulation and those responsive to the imposed stress [ 58].
However, our study is unique in that we have determined the temporal patterns of expression of the genes we identified.
While we were unable to analyze the spatial expression of specific sept9 isoforms we could, using RT-PCR, determine the temporal expression pattern of the various sept9 transcripts.
To determine the temporal expression pattern of ndg-4, we generated a transcriptional reporter strain expressing green fluorescent protein (GFP) under the promoter of ndg-4.
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