Sentence examples for determine the template from inspiring English sources
An intriguing issue concerning FFPE-DNA is to determine the template amount to be added per amplification reaction, which is particularly important for the evaluation of Q-PCR method efficiency.
16 The retrieved FASTA sequences for the target three capsid cores were submitted to protein basic local alignment search tool (pBLAST) to determine the template protein.
The number of amplification cycles needed to reach the crossing point (Cq) was used to determine the starting template amount in reactions where we assumed unknown template amount.
For each location of the window, moment features are used to determine the optimum template that is subsequently matched to the actual content of the window to produce a 'pattern intensity' map.
Curve fitting parameters were used to determine the minimal template DNA input required to elicit the cutoff value.
Standard PCRs were run with fivefold dilutions of the cDNA template to determine the optimal amount of template and optimal annealing temperature for the experimental and reference reactions, using 500 nM of each primer.
Thermogravimetric analysis (TGA) was used to determine the temperature for template removal.
The expression level of the housekeeping gene β2-microglobulin was measured as an internal reference with a standard curve to determine the integrity of template RNA for all specimens.
To determine the optimum target template to identify SNPs specific to single-copy sequences in tetraploid cotton, we tested primer pairs from available BAC-end sequences; sequences flanking SSRs or predicted introns in ESTs; and sequences in the 3' UTR or 5' terminus of ESTs (Table 1).
For example, whole genome sequencing can systematically identify CNV breakpoints and determine the source of the template for the repair and resolution of genetic exchange events.
To compare and determine the most accurate templating method, we retrospectively identified 33 patients between 50 and 83 years of age.
