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To determine the sphere formation capacity of treated tumours, dissociated cells were plated in 24-well non-tissue culture-treated plates in PM + CM containing 0.8% methylcellulose (Sigma) and sphere formation was scored after 10 days.
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The medium was renewed at day 3, and spheres were counted at day 7 to determine the sphere-forming capacity.
The association between the α and β subunits determines the sphere protein structure.
Furthermore, it outperforms all threshold methods regarding detection rate, determines the spheres' volumes more accurately than fixed threshold methods, and produces similar values as iterative thresholding.
Figure 5b shows the threshold levels needed to determine the correct sphere volume for 68Ga.
These methods split a biomacromolecular structure into slices along a start vector defined by the user and then optimization methods are used to determine the largest sphere.
This nearly ancient formula is still the easiest way to determine the surface area of a sphere.
A human observer study was performed to determine the smallest observable sphere for each scanning condition.
To determine the probability of spheres making contact with the loukoumasome by chance, we estimated the proportional volume of cytoplasm occupied by a 1 micron-thick layer coating the outside of the loukoumasomal torus.
The details of this balance determine whether the sphere will reach the membrane.
This very accurate control of the dispersive, polar and hydrogen contributions to the overall solubility now allows determining the Hansen sphere for P3HT and PCBM with much higher accuracy.
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