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where is the location of backbone node, the location of terminal node, the number of backbone nodes, the number of terminal nodes, the index of the backbone node covering terminal node, and the integer variables that determine the backbone topology (2).
Because the polypeptide backbone of a protein is a linked sequence of rigid planar peptide groups, these two angles essentially determine the backbone conformation of proteins.
The key bottleneck in these studies is to determine the backbone resonance assignment, which allows spectral peaks to be mapped to specific atoms.
Using C- and N-labeled Cdc5-D3, we ran a series of two-dimensional (2D) and three-dimensional (3D) NMR experiments to determine the backbone connectivity of Cdc5-D3.
Coupling constants measured from H−P HMBC spectra were applied to the Karplus relationship 74) to determine the backbone dihedral angle ε (C4′− C3′− O3′−P), related to the H3′− C3′− O3′−P angle by a 120° shift.
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Using heteronuclear NMR spectroscopy, we have determined the backbone (1 H, (13 C, and (15 N assignments for the pro-domain of PC1.
In each iteration, the net force acting on each backbone node is computed, which determines the backbone node's relocation direction.
We then successfully traced the second domain on a so-called mini-map, which was a stacked plastic sheet with electron density maps contoured in an old-fashion way to determine the proteins' backbone structure.
P H3′ J couplings were applied to determine the phosphodiester backbone conformation.P chemical shifts were referenced using indirect shift ratios.
Here we use a database of high-resolution protein crystal structures to determine the fundamental correlated backbone motions occurring in β-sheets.
As the purpose of the trial was to determine the best chemotherapy backbone for further study, none of the arms contained a true control.
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CEO of Professional Science Editing for Scientists @ prosciediting.com