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We used fibrin zymography to determine the activity of the plasminogen activators as previously described [48].
To determine the activity of RAS, the RAS activation assay kit (Upstate Millipore, Temecula, CA, USA) was used.
To determine the activity of this pathway we therefore stained for the phosphorylated activated form of Akt (pAktser473).
The entire VOI was used to determine the activity for 15O-water.
To determine the activity of SCR, the substrate succinate (20 mM) was used.
Analysis of variance was used to determine the activity of GABA-transaminase.
In clinical laboratories, glutamate is used to determine the activity of some aminotransferases.
The acoustic emission (AE) technique was concurrently applied to determine the activity of particular deformation mechanisms.
α-Amanitin was used to determine the activity of ribonucleic acid polymerase II.
Western blotting was used to determine the activity of intracellular extracellular regulated kinase (ERK1/2).
Several in vitro and in vivo assays were used to determine the activity of the analogs.
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