Nuclear (Fn) and cytoplasmic (Fc) fluorescence was determined subsequent to the subtraction of autofluorescence (also quantified), and used to derive the nuclear to cytoplasmic ratio (Fn/c), a parameter that enables differences in expression levels between individual transfected cells to be standardized.
The acetylcholinesterase (AChE electrical eel, Type VI-S, EC 3.1.1.7; Sigma) and butyrylcholinesterase (BuChE equine serum EC 3.1.1.8; Sigma) inhibitory activities of Ir.Cr were determined subsequent to minor modification of previously reported spectrophotometric method [ 42] by using 96-well plate reader Synergy HT, Biotek, USA, taking eserine (0.25 mM per well) as standard inhibitor.
Patients were followed up at their homes 28 days after enrolment to determine subsequent treatment seeking and any mortality or admissions to hospital, and to confirm socioeconomic variables.
Pain due to osteoarthritis is known to determine subsequent depressed mood through its effect on fatigue and disability.
While ASK1 is known to be activated by several internal or external environmental stressors, the extent and duration of the exposure to them is crucial to determine subsequent cell fate.
Shapiro-Wilk normality test was used to evaluate the normal distribution of mRNA expression data and to determine subsequent use of appropriate tests for statistical comparison.
Better yet, how do we avoid circular reasoning (i.e., using homology to determine the topology of the tree and then using the tree to determine subsequent homology)?
Fura-2 loaded cells in Ca2 +-free buffer were stimulated with TG (200 nM) to deplete ER Ca2 + stores, and then CaCl2 (2 mM) was added to determine subsequent Ca2 + entry (i.e. SOCE).
Anti-TNF anti tumor necrosis factor, PY patient-years While anti-TNF agents are the most widely used biologic drugs in RA, not all patients respond adequately, and limited data are available to rheumatologists to rationally determine subsequent therapy in anti-TNF experienced panti-TNF experienced
The most consistent criteria for recidivism applied in the literature uses official records to determine subsequent arrests and/or convictions for sexual and/or non-sexual offences.
