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Western blotting and immunohistochemistry were used to determine specificity of the VANGL1 antibody (Fig. S5).
To determine specificity of the antibody, positive controls were carried out on human cartilage tissue sections and negative controls were run on non-injected chick limbs.
To determine specificity of the PCR, a sample of human DNA from the LNCaP prostate cancer cell line (American Type Culture Collection, code CRL-1740) wamplifiedied with the XMRV and MLV primer sets.
Following amplification, melting temperatures of PCR products were analyzed to determine specificity of the PCR product.
To determine specificity of the primers, 51 isolates were also subjected to the LAMP assay.
To determine specificity of the SAFB2 knockout, SAFB1 cDNA spanning exons 1 through 4, and 9 through 11, was amplified.
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In addition to the Melt® procedure, migrating the PCR products on an agarose gel can also help in determining specificity of the amplification procedure.
For E. coli isoacceptors with overlapping codon specificity the parameters for the tRNA fraction that pairs to each codon were calculated according to the experimentally determined specificities of the ternary complexes [23], [24], [27], [52].
Finally, we determined specificity and sensitivity of the HER-2 domains' targeting by flow cytometry (Fig. 2).
To determine the specificity of the PAI-1 mRNA response, we then determined uPA and PAI-2 mRNA abundances.
To determine the specificity of the proposed method, a set of extract of blank milk samples (n = 20) were injected into the chromatographic system on the same day.
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