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We used this assumption to identify residues which determine specificity of protein DNA and protein ligand recognition.
Western blotting and immunohistochemistry were used to determine specificity of the VANGL1 antibody (Fig. S5).
To determine specificity of the antibody, positive controls were carried out on human cartilage tissue sections and negative controls were run on non-injected chick limbs.
To determine specificity of DNA binding, samples were incubated with or without 20 ng of unlabeled competitor DNA for 10 min at room temperature.
To determine specificity of the PCR, a sample of human DNA from the LNCaP prostate cancer cell line (American Type Culture Collection, code CRL-1740) wamplifiedied with the XMRV and MLV primer sets.
To determine specificity of VCAM-MPIO, a further group of mice (n = 2) was injected with 0.2 mg of VCAM-1 antibody per kg body weight to block VCAM-1 binding sites with subsequent administration of VCAM-MPIO 15 min later.
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The binding models were determined using HT-SELEX, the same method we have used previously to determine specificities of mammalian TFs.
In addition to the Melt® procedure, migrating the PCR products on an agarose gel can also help in determining specificity of the amplification procedure.
For E. coli isoacceptors with overlapping codon specificity the parameters for the tRNA fraction that pairs to each codon were calculated according to the experimentally determined specificities of the ternary complexes [23], [24], [27], [52].
To determine specificity, activity of selected substrates was characterized against proteinase 3 and neutrophil elastase.
The melting curve analysis was set to determine the specificity of candidate gene amplification.
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