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We followed standard post-run bioinformatics processing on the 454 platform to determine reads that passed various quality filters.
We also followed standard post-run, bioinformatics processing on the 454 platform to determine reads that passed various quality filters.
Standard post-run and bioinformatics processing on the 454 platform to determine reads that passed various quality filters were also performed following the manufacturer's recommendations http://www.454.com.
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At Huntington, incoming students undergo two to three hours of diagnostic and achievement testing to determine reading level, to measure phonetic ability, to assess ability to recall information and to pinpoint learning strengths and weaknesses.
In this paper, to avoid the dependency of stability parameters of SRAM cell to supply voltage (Vdd), here N-curve metrics has been analyzed to determine read and write stability.
For example, students were asked to design PCR primers, determine reading frames, and perform various calculations.
The best BLASTn [ 73] hit of individual reads against all contigs was used to determine read numbers per contig.
Post-sequencing analysis was performed using the WASP pipeline (v3.1.5 rev. 6632), involving read alignment using gsnap (2012-07-20), with htseq (v0.5.3p3) used to determine read quantitation.
SAMtools [ 75] was run on each BAM file to determine read coverage and coverage for each genomic base by each sample.
For RNA sequencing analysis, TopHat (Bowtie version 2.1.0) [ 5] was used to align reads to the human reference GRCh37-lite (accession id: GCA_000001405.1), and HTseq version 0.6.1 in "union" mode [ 6] was used to determine read counts.
To determine read counts matching each reference RNA at each step, a simple Perl script was written to scan each of the three SAM files and tally mapped read counts per transcript and then report them in tabular format.
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CEO of Professional Science Editing for Scientists @ prosciediting.com