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Positive Paneth cells were counted per crypt by a single blinded investigator to determine quantification.
Positive goblet cells were counted per 100 epithelial cells above the +4 position to determine quantification by a single blinded investigator.
RealPlex software v. 2.0 (Eppendorf) was used to determine quantification cycle (Cq) in non-amplified (CqNA) and amplified (CqA) total RNA samples.
Therefore, the technique has been investigated in the simple chemical and physicochemical processes of preconcentration, evaporation, an acid base reaction, crystallization, and with SERS solutions to determine quantification limits.
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In contrast, at the crux of technological genetic evaluation are Estimated Breeding Values or EBVs, statistically determined quantifications of genetic value.
We used a UV Vis spectrophotometer (NanoDrop 2000c, USA) to determine DNA quantification and quality.
NP cytotoxicity was also possible to determine by quantification of cell apoptosis.
We applied qPCR to determine relative quantification of genomic DNA between the two strains before and after competition.
Briefly, this method uses arithmetic formulas to determine relative quantification.
The standard curve method was used to determine relative quantification of mRNA abundance [ 27].
Firstly, conditions in which proliferation was maximal had to be established to determine accurate quantification of these cells.
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