Sentence examples for determine prion from inspiring English sources

However, the relative distance between FDCs and peripheral nerves [39], and PrPC expression in the peripheral nervous system [40] determine prion neuroinvasion efficiency and onset of terminal disease.

In order to further confirm the association of prion infectivity with membrane-derived MVs and to determine prion titres, in vivo biossay was performed in which purified MVs from infected and non-infected Neuro-2a PK1 cells or cell lysates were intracerebrally inoculated into tga20 indicator mice [49].

However, it appears that the polymorphic codon V is associated with prion positivity of all nuclei more than PrPSc type 2. In fact, the presence of PrPSc type 2 appeared neither sufficient nor necessary to determine prion protein deposition as demonstrated by MM2 cases (case 11 and case 12).

This emphasizes the decisive role of peripheral immune system components in determining prion susceptibility.

While the conformational change from the α-fold to the β-sheet rich state determines prion amplification properties, the role played by methionine oxidation in the global conversion process remains unknown.

We therefore applied this protocol to both uninfected CD-1 and RML prion-infected brain homogenate before or after digestion with 100 µg/ml pronase E at 37°C for 30 min and subsequently measured PrP concentration by ELISA or immunoblotting and determined prion infectivity using the SCA.

By determining prion propagation rates of a thousand PK1 subclones, three revertant clones (R2, R5, and R7) showed markedly reduced prion propagation rates when compared to susceptible PK1 cells (Fig 1B).

By making use of well-characterized genetic assays determining prion-like characteristics of glutamine-rich domains in different proteins, we have identified the Q domains of both the GAF isoforms as prion-like domains.

To determine if Hsp104 localization to heat-induced aggregates rather than their accumulation per se determined prion-curing efficiency, we replaced endogenous HSP104 with an HSP104-GFP fusion, which supports [ PSI + ] propagation.

Samples were retrieved after digestion, were neutralized and inoculated intracerebrally into transgenic mice expressing the cervid protein to determine remaining prion infectivity.

To determine BSE prion inactivation during the manufacturing process of MBMs, industrial processes were replicated on a pilot scale by using BSE-infected brains, and the infectivity of processed materials in each step was investigated in detail [ 9].