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We were able to anchor 99 scaffolds with 2 or more SNPs and thus determine orientation for over 327 Mb (90.5%) of the scaffold assembly.
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The approach is promising in that it uses flow solution features, rather than grid features, to determine the orientation for the solution method.
By using strand-specific reads, we were able to determine the orientation for all nTARs.
However, to determine whether the observed IS orientation biases could just reflect a general preference for insertion in a specific orientation or the effect of selection, we determined global orientation for non-IS encoded and IS-encoded genes in 1,727 bacterial chromosomes (fig. 2).
Rnnotator also determines the orientation for each transcript.
We have performed the requested additional experiments and determined microtubule orientation for uMAP4 and mMAP4 depletion as well as for the oMAP4 rescue experiment.
Distribution histograms were generated by determining the orientations for individual stereociliary bundles relative to a parallel line to the neural-abneural axis.
Keeping in mind that the driver face database is composed of face examples for five different orientations, the face is passed to the neural network to determine its orientation, specifically for the up and down cases.
FREALIGN was first run for one cycle using mode 3 (systematic parameter search) with an angular step of 7° to determine initial orientation parameters for each particle relative to the starting model.
Additionally we have been able to determine the optimal orientation for transgene expression at the ROSA26 locus.
After obtaining n segmented leaves (n ≥ 2), we determine the leaf orientation for each leaf with a "leaf orientation line" which is the longest line across the leaf.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com