Sentence examples for determine library from inspiring English sources

The library quality assessment used test ligations to determine library titer.

We suggest assessing all reads in the <17Kb MT genome to determine library quality: are reads the size that is expected?

A serial dilution of the resulting culture was used to determine library size (~106 mutants) and DNA sequencing and a preliminary TMDH analysis were used to demonstrate that the transposon had integrated throughout the S. aureus chromosome (see Figure 2).

Are all C residues converted to T? Beyond visual assessments, we also generated data at several imprinted regions that can be used by any other group using brain DNA to determine library quality.

We determined library concentration on a Nanodrop spectrophotometer (NanoDrop products, Wilmington, DE) and hybridized 4 pM of adaptor-ligated DNA to the flow cell.

Thus, while DNA concentration plays a critical role determining library proportion of MVT (see above), it appears that MVT proportions are independent of overall transformation efficiency.

We assessed the library quality by assaying ligations and carrying out 5′-end sequencing; the former procedure determined library titer, and the latter used to evaluate cDNA full-length percentage as well as the proportion of empty vectors.

Quality control for the final library was performed by measuring the DNA concentration with the QuBit dsDNA HS assay (Life Technologies, Q32851) on QuBit 2.0 Fluorometer (Life Technologies, Q32866) and by determining library fragment sizes with the Experion DNA 1K Analysis kit (Bio-Rad, 700-7107) on the Experion Automated Electrophoresis Station (Bio-Rad, 701-7000).

Three hundred microliters of cells from subtraction library was removed for plating assays (100 μl for each assay) to determine subtraction library capacity before 1 ml of transformed cells was inoculated in 10 ml of LB/Amp medium for overnight amplification.

The final libraries were assessed for quality with the Bioanalyzer (Agilent, Santa Clara, CA) to determine final library size and concentration.

Additional serial dilutions were plated to determine the library size.