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However, the incubation of cells, tissues or the whole plant in a stable isotope enriched environment and the use of either mass spectrometry or nuclear magnetic resonance techniques to determine label incorporation within specific metabolites offers the possibility to readily obtain metabolic information with higher resolution.
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To determine labeling indices, a minimum of 1000 cells/dish were scored.
Peptides were extracted into ACN and were ready for analysis by LC-MS/MS to determine labeling rates.
With method 1, gold counts are combined with stereological estimators of compartment size to determine labelling density (LD).
To determine labeling indices, 1,000 total cells in at least five randomly chosen microscopic fields in divergent regions of each carcinoma were counted by an investigator blind to the experimental status.
Users can calibrate the software with their own visually determined labeling index data and derive a suitable result correction equation (a third degree polynomial).
Supplementary Table S-3 shows calculated %SASA and the experimentally determined labeling results at five concentrations of PGO, ranging from 0.5 to 7 mM.
The "response to intervention" approach seeks to account for this and some argue that an "assessment for intervention" is more adequate than an "assessment for determining labels" [ 34, 35].
However, flux measurements – except for exchange reactions that deliver metabolites into the external space – are still difficult and costly to perform as they require determining labeled isotopomers in a time-dependent manner [ 11- 13].
In order to determine the label half-life and transit-time of STAT, S is both, the initially labeled entity and the target pool.
Thirdly, the nonparametric skew is utilized to determine the label of the period.
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